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Mobio 土壤DNA提取试剂盒(视频)

发布者:信息部    发布时间:2015-05-06


土壤、粪便等各种环境样品中提取出不含PCR抑制因子的高质量的微生物基因组DNA


品牌        货号 提取次数          
Mobio              12888-50            
  50次                     

相关产品

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腐植酸PCR抑制因子去除试剂盒


产品特点

・         从土壤、粪便等复杂环境样品中提取不含抑制因子的高质量DNA

・         专利技术100%去除腐植酸等PCR抑制因子

・         只要30min就能快速提取250mg土壤样品DNA


描述

MO BIO的强力土壤DNA提取试剂盒(PowerSoil® DNA Isolation Kit)使用了专利的抑制因子去除技术(Inhibitor Removal Technology®),能提取所有类型土壤,以及包括粪便、肠内容物、生物固体等环境样品的微生物总DNA。堆肥、底泥沉积物、粪肥等样品的最终DNA纯度非常高,可直接用于所有PCR、QPCR等下游实验。PCR分析已经证明可成功提取所有类型环境微生物,包括格兰性阴阳性菌(如芽孢杆菌、炭疽杆菌)、真菌(如酵母、霉菌)、藻类、放线菌(如链霉素)和线虫等。PowerSoil的专利腐殖质/棕色物质去除流程能去除所有复杂土壤类型中的各种PCR抑制因子,保证最终DNA能顺利进行PCR、qPCR、新一代测序等各种高要求下游应用,因而使用效果比其它品牌试剂盒更加出色。


研究与深入

强力土壤DNA提取试剂盒(PowerSoil® DNA Isolation Kit)非常适合PCR方法做土壤微生物多样性分析

 

使用MOBIO的强力土壤DNA提取试剂盒(PowerSoil DNA Isolation Kit),及其他两家厂商的产品提取9个不同土壤样品总基因组DNA。所有操作严格按照厂商提供说明书来进行。所得DNA 作0.8%TAE琼脂糖凝胶电泳显示如上(每孔15μl)。


操作演示视频


已发表部分参考文献

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・ Yan Li, Gary W. Lawrence, Shien Lu, Clarissa Balbalian , and Vincent P. Klink.Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production ;PLoS One. 2014; 9(2): e89887. 
・ Daniel I. Bolnick, Lisa K. Snowberg, Philipp E. Hirsch, Christian L. Lauber,Elin Org,Brian Parks, Aldons J. Lusis,Rob Knight, Gregory Caporaso,and Richard Svanback. Individual diet has sex-dependent effects on vertebrate gut microbiota ;Nat Commun. 2014 Jul 29; 5: 4500. 

・ Mummey, D.L., Rillig, M.C.The Invasive Plant Species Centaurea maculosa Alters Arbuscular Mycorrhizal Fungal Communities In The Field .Plant Soil. 288: 81-90 (2006).


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